Pdf normal anatomy and histology of the adult zebrafish. To only access descriptive diagrams detailing the wiring patterns of zebrafish vessels click the left button below. Anatomy, animal gross anatomy, fish anatomy, gross anatomy. Anatomy of the 24, 48, 72 and 120 hours zebrafish danio rerio embryo. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype. Initially blood flows through a simple single circulatory loop 24 hpf overview. Feb 25, 2014 the zebrafish anatomy ontology zfa is an obo foundry ontology that is used in conjunction with the zebrafish stage ontology zfs to describe the gross and cellular anatomy and development of the zebrafish, danio rerio, from single cell zygote to adult. Phrase searching you can use double quotes to search for a series of words in a particular order.
On the home page menu click on search anatomical terms. Neuroanatomy of the zebrafish brain a topological atlas mario f. More precisely, zebrafish belong to the single largest vertebrate family. Chemical anatomy of the zebrafish retina springerlink. This fullcolor atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish. If you have ever heard of the show or watched it, you must know that it is aimed at medical students and it. The zebrafish has been shown to be an excellent vertebrate model for studying the roles of specific genes and signaling pathways. Science education collection an introduction to the. To view an anatomical part within the context of a virtual slide, choose a term from the list below. The zebrafish international resource center is housed on the university of oregon campus in eugene. An online database of zebrafish anatomy article pdf available in bmc biology 51. Zfin the 120 hr embryo zebrafish information network.
Although human pathologies have mostly been modeled using higher mammal systems such as mice, the lower vertebrate zebrafish has gained tremendous attention as a model system. A structured controlled vocabulary of the anatomy and development of the zebrafish danio rerio. This poster is a valuable reference tool, featuring illustrations on the zebrafish anatomy. Although experimental analysis of the embryo and larva is extensive and the morphology has been well documented, descriptions of adult zebrafish anatomy and studies of development of the adult structures and organs, together with techniques for working with adults are lacking. This collection of sections through zebrafish embryos at four different stages of development is thought to provide some help to understand how the zebrafish embryo looks inside. Click download or read online button to get atlas of zebrafish development book now. The deltaa gene of zebrafish mediates lateral inhibition of hair cells in the inner ear and is regulated by pax2.
Aug 27, 20 is an online neuroanatomical atlas of the embryonic zebrafish. Native to south asia, it is a popular aquarium fish, frequently sold under the trade name zebra danio and thus often called a tropical fish although both tropical and subtropical. Animated images in the analysis of zebrafish behavior. A novel integrated resource to facilitate zebrafish research. We have used confocal microangiography to examine and describe the vascular anatomy of the developing zebrafish, danio rerio. Download grays anatomy for students pdf free latest edition. Cyprinidae, which contains over 2,400 species, including the loveable goldfish.
Dissection of organs from the adult zebrafish protocol. Wildcard searching if you want to search for multiple variations of a word, you can substitute a special symbol called a wildcard for one or more letters. Guidance on the housing and care of zebrafish, danio rerio. Danio rerio commonly known as the zebrafish is a tropical fish belonging to the minnow family cyprinidae, commonly kept in aquaria and used for scientific research. A structured controlled vocabulary of the anatomy and development of the zebrafish. A lifespan atlas of the zebrafish bioatlas bioatlas. The latest edition which is the second edition was published in 2009 by elsevier. The sequencing of its genome and the relative ease with which gene modifications can be performed have led to the creation of numerous human disease models that can be used for testing the potential and the toxicity of new pharmaceutical compounds. This article is from journal of biomedical semantics, volume 5. The zebrafish danio rerio is a valuable and common model for researchers working in the fields of genetics, oncology and developmental sciences. With the credentials of zebrafish as a developmental model now well recognized, the emerging new opportunity is the wider application of zebrafish biology to aspects of human disease modelling. Zebrafish study reveals first fine structure of a complete. The gut microbiota plays a crucial role in the bidirectional gutbrain axis, a communication that integrates the gut and central nervous system cns activities. The drawings illustrate the critical embryonic stages and details the adult anatomy, including external views of the male and female lateral and male ventral, skeleton, viscera of the female and male lateral, and male ventral, internal organs, central nervous system, histological views of male and.
Probiotic modulation of the microbiotagutbrain axis and. The development of tools to predict gene expression from literature analysis. A guide for the laboratory use of zebrafish danio rerio, eugene, university of oregon press. Anatomy this poster portrays the critical embryonic stages and the details of adult anatomy, including aspects of sexual dimorphism. An introduction to zebrafish jove journal of visualized. Anatomical dissection of zebrafish brain development. Neurochemical anatomy of the zebrafish retina as determined by immunocytochemistry. Online help keyboard shortcuts feed builder whats new. Anatomy atlases and resources general information zfin. The behaviour and ecology of the zebrafish, danio rerio. Staging by externally visible anatomy of the living fish. The 120 hr embryo dorsal view click on a number to follow the appropriate link lateral view. For example, world war ii with quotes will give more precise results than world war ii without quotes.
The atlas spans the life of the zebrafish from embryo to. In the following sections, we describe the anatomy and physiology of the zebrafish cornea and lens. The zebrafish danio rerio is a freshwater fish belonging to the minnow family of the order cypriniformes. Zebrafish vascular anatomy introduction of the interactive. Anterior is to left in this and other figures in the paper except where indicated. Materials and zebrafish strains are distributed to the research community. Weinstein,1 laboratory of molecular genetics, nichd, nih, building 6b, room 309, 6 center drive. High resolution confocal imaging of intact brains in which. Use the autocomplete tool to find the term that you want to use. Oct 15, 2019 the zebrafish virtual atlas and the zebrafish anatomy project the zebrafish atlas contains 2 and 3dimensional, anatomical reference slides of zebrafish to support research and education worldwide serial histological images of wild type zebrafish are used for 3d reconstructions. Over the last two decades, zebrafish have been established as a genetically versatile model system for investigating many different aspects of vertebrate developmental biology. Review 1 the behaviour and ecology of the zebrafish, danio rerio rowena spence1, gabriele gerlach2, christian lawrence3 and carl smith1 1 department of biology, university of leicester, university road, leicester, le1 7rh, uk. Learn about human anatomy and physiology online by downloading openstaxs free anatomy and physiology book and using our accompanying study guide.
The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data from the. In our lab we use the zebrafish for cancer research, and it is the basis for this histological atlas. For example, mutant zebrafish are being utilized for testing drugs that will combat a range of human diseases, from alzheimers and cancer to kidney failure and congenital heart disease. Danio rerio are among the smallest members of this family, with adults measuring 30 40 millimeters, or about 1. Zebrafish danio rerio have become an increasingly popular genetic model over the past twenty years.
The zebrafish anatomy poster drawings were done by gheorghe m. Zebrafishbrain information about the neuroanatomy of the developing zebrafish brain. If you want to view 3d angiography images, click the right button below. This collection of sections through zebrafish embryos at four different stages of. The sequencing of its genome and the relative ease with which gene modifications can be performed have led to the creation of numerous human disease models that can be used for testing the potential and the toxicity. Provide a portal for improved searching of other information sources. Blood exits the heart through the bulbus arteriosus and the ventral aorta then branches to the right and left into the mandibular first aortic arches. The zebrafish anatomy portal zfap is an anatomical resource for the zebrafish danio rerio containing 3d models of zebrafish covering the range of development from 24hrs until adulthood. Richardson technological innovation has helped the zebrafish embryo gain ground as a disease model and an assay system for drug screening. For further description see schilling and kimmel 1997 and cubbage and mabee 1996. Zebrafish anatomy and development ontology ncbo bioportal.
A molecular phenotype atlas of the zebrafish retina. Author links open overlay panel david salgado b christophe marcelle b peter d. Methods to study the development, anatomy, and function of. Aug 17, 2007 over the last two decades, zebrafish have been established as a genetically versatile model system for investigating many different aspects of vertebrate developmental biology. Thus, the primary form of mercury to which humans are exposed is methylmercury. Zebrafish get their name because they resemble zebras.
Zebrafish study reveals first fine structure of a complete vertebrate brain 11 may 2017 zebrafish larva head in crosssection. Internal organs are illustrated in multiple dissections. The growing interest in zebrafish research was paralleled by an increase in tools and methods. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data from the primary literature. The vertebrate cornea is the transparent tissue covering the. The zebrafish anatomy ontology zfa is an obo foundry ontology that is used in conjunction with the zebrafish stage ontology zfs to describe the gross and cellular anatomy and development of the zebrafish, danio rerio, from single cell zygote to adult. A virtual slide, with capabilities to zoom and scroll, illustrates the term. During development of the zebrafish embryo, the ear undergoes dynamic morphogenesis from a simple epithelial vesicle into a complex labyrinth, consisting of three semicircular canals and three otolithic sensory organs, each with an array of differentiated cell types. The study of zebrafish as a leading model organism for developmental biology is rapidly expanding. Search for annotated images illustrating structures of interest. Anatomy atlases and resources zebrafish information network.
Grays anatomy for students pdf is a book written by adam mitchell, richard drake, and wayne vogl. Thin section in araldite were stained with methylene blue. Zebra fish, any member of either of two unrelated groups of fishes, the freshwater species in the genus brachydanio family cyprinidae. Users can view threedimensional fluorescent angiograms of any region of the embryo at any stage of development from 17 days postfertilization. Constantinescu, dvm, phd, drhc, a veterinary anatomist and medical illustrator at the university of missouricolumbia. Generation of disease models and drug screens shaukat ali, danielle l. An atlas of embryonic and early larval development sumio isogai, masaharu horiguchi, and brant m. Normal anatomy and histology of the adult zebrafish.
This website is an online interactive atlas displaying in threedimensional detail the complete vascular anatomy of the developing zebrafish. Free anatomy and physiology textbook for download openstax. About zfap the zebrafish anatomy portal allows users to. This method and the profound optical clarity of zebrafish embryos make it possible to view the entire developing vasculature with unprecedented resolution. The zebrafish virtual atlas and the zebrafish anatomy project the zebrafish atlas contains 2 and 3dimensional, anatomical reference slides of zebrafish to support research and education worldwide serial histological images of wild type zebrafish are used for 3d reconstructions. The atlas uses highresolution confocal images and movies of transgenic lines to describe different brain structures. Its mission is to provide a central repository for wildtype and mutant strains of zebrafish danio rerio and for materials and information about zebrafish research. The course is held either in english or in german depending on. This section offers an interactive reference to each anatomical term identified in the zebrafish atlas. We define seven broad peri ods of embryogenesisthe zygote, cleavage, blas. For the first time, this atlas provides the research community with a complete reference for zebrafish anatomy spanning the early embryo all the way to adulthood. The zebrafish model organism database zfin uses the zfa and zfs to annotate phenotype and gene expression data.
This site is like a library, use search box in the widget to get ebook that you want. Interestingly, unlike in prior studies where zebrafish embryos were exposed to higher doses of alcohol and for longer periods of alcohol administration e. Atlas of zebrafish development download ebook pdf, epub. The visual system of zebrafish and its use to model human ocular. This fullcolor atlas will aid experimental design and interpretation in these areas by providing a fundamental understanding of zebrafish anatomy. Whilst the vascular anatomy of the embryonic fish, the anatomy of the embryonic and adult brain 11,12, and heart have been elegantly described, there is a need for a detailed record of zebrafish development that spans the entire life history of the organism and presents its anatomy.
This links you to a page from which you can directly load different diagrams of vessels in embryos from 17 days old. This chapter covers detail of materials and protocols that we employ to generate data for the atlas. Mar 09, 2016 the inner ear is a remarkably intricate structure able to detect sound, motion, and gravity. The zebrafish atlas of macroscopic and microscopic anatomy the zebrafish danio rerio is avaluable and common model for researchers working in the fields of genetics, oncology, and developmental sciences. Growth of the zebrafish anatomy ontology expanded to support adult morphology and dynamic changes in the early embryo ceri e. Normal table of postembryonic zebrafish development. Provide the ability to search images by anatomical terms. The advantages of zebrafish over classical vertebrate models are multifactorial and include high genetic and organ system homology to humans, high fecundity, external fertilization, ease of genetic manipulation, and. Use of zebrafish danio rerio embryos as a model to assess effects. A template for the creation of anatomical resources for other model species. Development of utricular otoliths, but not saccular otoliths, is necessary for vestibular function and survival in zebrafish. Highlights we describe a novel user friendly anatomical resource for zebrafish.
1503 555 1483 402 274 616 1466 1355 74 933 53 121 587 821 566 1259 1503 81 295 25 747 1186 87 1325 658 965 584 687 926 137 860 891 455 591 1002 1476 1208 142 1468 399 761 236 1199 369